Conjugated Dienes Analysis Essay

Free-radical chain reactions, which take place during lipid peroxidation, lead to the formation of lipid hydroperoxides that decompose to many secondary products. Among the products, is a three-carbon dialdehyde, malonaldehyde. When ribonuclease reacts with peroxidizing polyunsaturated lipid and with malonaldehyde, it becomes inactivated and yellow fluorescent products are formed. The spectral properties of these chromophoric systems are nearly identical with those in chloroform–methanol extracts of age pigment (lipofuscin). Fluorescent product formation also occurs in peroxidizing subcellular particles, amino acid and phospholipid systems, peroxidizing arachidonic acid and DNA, and antioxidant-deficient mouse and rat tissues. A major portion of fluorescent molecular damage in biological tissues is found in the lipid-soluble phase of chloroform–methanol extracts. The simple method described is for the extraction and measurement of fluorescent molecular damage products of lipid peroxidation from animal tissues.

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